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A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba
Delineation Paralleled Genotyping
2015/4/1
Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important
food and feed grain crop legume, they have not yet been used for comprehensive molecula...
微卫星PCR产物变性与非变性PAGE-银染检测方法的比较The Comparision of Denatured and non-denatured PAGE–Silver- Staining Detection of PCR Product of Microsatellites
微卫星 PAGE PCR 银染
2008/1/8
摘要
用鸡的微卫星引物对6个中国地方鸡种的两个微卫星位点进行PCR扩增。将扩增产物在变性与非变性聚丙烯酰胺凝胶（PAGE）上进行电泳，经银染，表明微卫星产物在二者上的电泳结果有明显的差异。在非变性聚丙烯酰胺凝胶中，表现为有较多的非特异带，而在变性胶中微卫星扩增产物条带清晰，易于鉴定。Abstract：The genomic DNAs from six chicken breeds in Chin...
栓皮栎天然群体SSR遗传多样性研究Genetic Diversity of Microsatellites（SSRs）of Natural Populations of Quercus variabilis
栓皮栎 微卫星(SSR) 遗传多样性 遗传分化
2008/1/8
摘要
利用微卫星(SSR)标记对我国4个省内的5个栓皮栎（Quercus variabilis Bl.）天然群体的遗传多样性进行了研究。16对SSR标记揭示了栓皮栎丰富的遗传多样性：等位基因数（A）平均8.4375个，有效等位基因数(Ne)平均为5.9512个，平均期望杂合度(He)0.8059，Nei多样性指数(h)为0.8041。栓皮栎自然分布区中心地带的群体具有较高的遗传多样性，而人为对森...
摘要微卫星序列广泛存在于各类真核生物基因组中，一般为散在分布的中等程度重复序列。不同物种中，微卫星序列的含量以及占优势的微卫星序列类型各不相同。复制时,微卫星序列易于发生长度突变，这种突变与微卫星序列的复制滑移有关，同时也受多种因素的影响。微卫星序列可能是原微卫星序列通过复制滑移使序列长度扩增形成的。进化过程中，微卫星序列的长度变化维持在一定的范围内。由于微卫星标记多态性高、重复性好，并且操作简单...
Molecular Characterisation of Nilagiri Sheep (Ovis aries) of South India Based on Microsatellites
Genetic Characterisation Nilagiri Sheep Microsatellites PIC Inbreeding Estimates
2016/4/25
Genetic variation in Nilagiri sheep, the only apparel wool breed in South India was studied using 25 FAO recommended ovine-specific microsatellite markers. The number of observed alleles ranged from 3...
Genetic resources of barley and oat characterised by microsatellites
barley oat microsatellites genetic diversity
2014/3/13
Barley (Hordeum vulgare L.) and oat (Avena sativa L.) are important crop species. 1865 accessions of winter barley, 2707 accessions of spring barley and 1998 accessions of oat are maintained in RICP G...