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Formation and Analysis of Topographical Domains Between Lipid Membranes Tethered by DNA Hybrids of Different Lengths
Cell Membrane DNA Membrane Lipids Lipid Bilayers 1,2-Dipalmitoylphosphatidylcholine Microscopy, Fluorescence Base Sequence Molecular Sequence Data Models,Chemical Unilamellar Liposomes
2016/5/25
We recently described a strategy to prepare DNA-tethered lipid membranes either to fixed DNA on a surface or to DNA displayed on a supported bilayer [Boxer et al., J. Struct. Biol., 2009, 168, 190; Bo...
Early Steps of Supported Bilayer Formation Probed by Single Vesicle Fluorescence Assays
Adsorption Diffusion Lipid Bilayers Lipid Bilayers Liposomes Membrane Fluidity Membrane Fusion Membranes,Artificial Microscopy,Fluorescence,Multiphoton Motion Phosphatidylcholines Phosphatidylserines Xanthenes
2016/5/24
We have developed a single vesicle assay to study the mechanisms of supported bilayer formation. Fluorescently labeled, unilamellar vesicles (30-100 nm diameter) were first adsorbed to a quartz surfac...
Micropattern Formation in Supported Lipid Membranes
Lipid Bilayers Phospholipids Microchemistry Diffusion Membrane Fluidity Electrochemistry
2016/5/24
Phospholipid vesicles exhibit a natural tendency to fuse and assemble into a continuous single bilayer membrane on silica and several other substrate materials. The resulting supported membrane mainta...
Formation of Supported Lipid Bilayer Composition Arrays by Controlled Mixing and Surface Capture
tetrafluorophosphite mean amplitudes of vibration bond properties
2016/5/23
Virtually all structures in living cells are defined by lipid bilayers. Many molecular components can diffuse along these 2-dimensional structures, sampling all or part of the membrane, and this diffu...
Formation and Spreading of Lipid Bilayers On Planar Glass Supports
Formation Spreading Lipid Bilayers Planar Glass Supports
2016/5/23
The fusion and spreading of phospholipid bilayers on glass surfaces was investigated as a function of pH and ionic strength. Membrane fusion to the support was favorable at high ionic strength and low...