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An MspI PCR-RFLP detecting a single nucleotide polymorphism at alpha-lactalbumin gene in goat
goat alpha-lactalbumin gene polymorphism Single Nucleotide Polymorphism (SNP) PCR-RFLP
2015/6/4
Alpha-lactalbumin (α-LA, LALBA) was strongly correlated with the nutritional value and the functional properties of whey and whey products. However, there are not many studies of goat LALBA gene and i...
南阳牛DGAT2基因PCR-RFLP多态性及其与生长性状相关性研究
DGAT2 南阳牛 PCR-RFLP 生长性状
2008/1/12
摘要以131头纯种南阳牛为研究材料, 利用PCR－RFLPs对二酰甘油酰基转移酶2(DGAT2)基因的第6内含子和第7内含子的多态性及其与生长发育的相关性进行了分析。结果表明: 南阳牛在该位点分别检测到两种等位基因A/B和N/M, 频率分别为0.875/0.125和0.971/0.029。A和N等位基因是群体中的优势等位基因。该基因内含子6对南阳牛6月龄的体高、2岁体重、6月龄到两岁的胸围和体斜长...
HLA-DRB1基因位点多态性的PCR-RFLP分析
人类白细胞抗原 ＤＲＢ１基因 聚合酶链反应 限制性片段长度多态性
2007/12/18
摘要 设计并建立一套适合国内应用的改良ＰＣＲ－ＲＦＬＲ方法，分５组特异性扩增ＤＮＡ样品，随后进行酶切定型分析，准确检测了编码ＤＲ抗原特异性的ＨＬＡ－ＤＲＢ１基因位点的多态性，该法采用分组扩增，不发生与其它ＤＲＢ位点等位基因的交叉扩增，不仅适合纯合子的区分而且可以清楚准确地检测杂合子样品，已报道过的ＤＲＢ１位点编码的特异性组合都可以通过这个方法得到准确分析。所使用的Ⅱ类限制性内切酶均价格便宜、易购。...
用PCR及RFLP方法分析支原体与植原体的同源性
支原体 植原体 PCR RFLP
2007/8/5
题目：用PCR及RFLP方法分析支原体与植原体的同源性
作者：邓兆群胡勤学 柳 晟 王 瑶 林木兰
湖北医科大学微生物及免疫学教研室，武汉
摘要：
将猪鼻支原体和泡桐丛枝病植原体的16S rDNA进行PCR扩增，分别得到一条1 kb左右的扩增片段。PCR扩增产物用限制性内切酶EcoRⅠ、HindⅢ、BamHⅠ、SalⅠ和SmaⅠ进行RFLP(限制性片段长度多态性)分析，发现用RFLP分析猪...
Association between PCR-RFLP Polymorphisms of Five Gene Loci and Milk Traits in Chinese Holstein
Chinese Holstein beta-lg kappa-cn IGFBP-3 Milk Traits
2016/4/25
The objective of this study was to assess the association of polymorphisms in 觀-cn, ??lg, ??lg 5??flanking region, CSN1S2, and IGFBP-3 genes with milk production traits and mastitis-related traits in ...
Genetic Relationships of Cattle Breeds Assessed by PCR-RFLP of the Bovine Mitochondrial DNA D-loop Region
Bos Taurus Bos Indicus Mitochondrial DNA PCR-RFLP Genetic Distance
2016/4/20
To investigate the genetic relationships among various cattle breeds, bovine mtDNA D-loop region was used in 411 animals of 18 cattle breeds, including 8 Asian Bos taurus, 7 European Bos taurus, 1 Asi...
Association between PCR-RFLP Polymorphism of the Fifth Intron in Lipoprotein Lipase Gene and Productive Traits in Pig Resource Family
Association Productive Traits Polymorphism Lipoprotein Lipase Gene Pigs
2016/4/19
The study was aimed at detecting polymorphism of the fifth intron in lipoprotein lipase (LPL) gene and analyzing association between the polymorphism and productive traits. A pair of primers was desig...
Three-step PCR and RFLP Genotyping of the Swine Ryanodine Receptor Gene Using Aged Single Hair Follicles Delivered by General Mail
Three-step (Booster and Nested) PCR Amplification RFLP Analysis Aged Hair Follicles RYR 1 Locus Genotyping
2016/4/11
We have developed a reliable and noninvasive method for swine genotyping of single locus nuclear gene with aged single hair follicles delivered by general mail. The method is based on booster and nest...