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南京农业大学农学院《The Plant Journal》发表油菜遗传育种团队“Mutation to a cytochrome P450-like gene alters the leaf color by affecting the heme and chlorophyll biosynthesis pathways in Brassica napus”（图）
油菜遗传育种 叶绿素 生物合成
2023/10/28
2023年7月28日，The Plant Journal在线发表了南京农业大学作物遗传与种质创新利用全国重点实验室油菜遗传育种团队的最新研究成果“Mutation to a cytochrome P450-like gene alters the leaf color by affecting the heme and chlorophyll biosynthesis pathways in Br...
细胞色素P450(以下简称CYP)与昆虫的抗药性密切相关。本研究运用AutoDock分子对接技术和分子力学泊松-波尔兹曼表面积法(molecular mechanics Poisson-Boltzmann surface area,MM-PBSA)结合自由能计算方法,分析了甜菜夜蛾CYP9A11与3种杀虫剂结合的作用位点、作用力类型和大小。结果表明:CYP9A11与毒死蜱结合形成两个氢键,有8个氨...
This study was conducted to investigate the effect of the ergot alkaloid, ergotamine (ET), on the induction of CYP3A and the interaction in vivo and in vitro with ET. Sprague-Dawley rats were treated ...
蓖麻细胞色素P450基因片段的克隆及RNAi表达载体的构建
蓖麻 P450 RNAi 载体构建
2013/9/5
采用PCR方法克隆了蓖麻细胞色素P450基因的正反2个片段。正向片段长为525个碱基,反向片段长为421个碱基。经测序分析,得到的正向基因片段和反向基因片段与GenBank 上的蓖麻细胞色素P450基因(XM_002525863.1)的碱基同源性分别为98.85%和99.51%。利用载体pHANNIBAL和限制性内切酶将正反2个片段连接成具有发夹结构的中间载体pHAN-P450S-P450A,然后...
Liver cytochrome P450 system as affected by endophyte-infected tall fescue seed extracts and ergot alkaloids
Seed Extract Tall Fescue CYP3A4 Ergot Alkaloid
2013/2/27
Endophyte infected tall fescue (E+) is the base diet for nearly all beef cattle in the southern USA. It has been linked to a variety of toxicological conditions due to the presence of large numbers of...
为了研究皇冠草基因组内的细胞色素P450基因或与P450基因相似的序列，根据哺乳动物细胞色素P450基因设计8条引物，以皇冠草总DNA为模板，进行PCR扩增。结果表明：共获得了26条扩增片段，片段长度300~1500 bp；将其中三条片段进行测序，BLAST分析表明这些序列与已发表的16种植物的细胞色素P450基因具有一定的相似性。这表明在皇冠草基因组内存在P450基因或者有与P450基因相似的序...
采用反转录多聚酶链式反应(RT-PCR)技术，从甜菜夜蛾Spodoptera exigua (Hübner)雄蛾触角内扩增得到2个P450基因cDNA片段CYP4L15和CYP4L16。序列分析表明，两个片段均具有P450的保守区序列，并且与几种已知参与气味降解的P450具有较高的同源性。通过RT-PCR方法进行的组织表达谱分析表明，2个基因在雄蛾触角和足内高量表达，在头部和胸部低量表达；此外，C...
Cloning of a Cytochrome P450 Gene Induced by Ethylene Treatment in Deepwater Rice (Oryza sativa L.)
Cytochrome P450 Deepwater rice Differential display Ethylene Internode elongation
2009/7/21
Cloning of a Cytochrome P450 Gene Induced by Ethylene Treatment in Deepwater Rice (Oryza sativa L.)