搜索结果: 1-6 共查到“植物病毒学 ELISA”相关记录6条 . 查询时间(0.106 秒)
The Production of Antiserum against Myrobalan Latent Ringspot Virus for Detection of the Virus using ELISA
nepovirus MLRSV purification immunisation of rabbits
2015/3/2
Myrobalan latent ringspot virus (MLRSV) was purified from the extract of infected Chenopodium quinoa Willd.,
using n-octanol, differential centrifugation, and saccharose gradient centrifugation. Ele...
番茄黄化曲叶病毒双抗体夹心ELISA检测方法的初步建立
番茄黄化曲叶病毒 DAS-ELISA 快速检测
2013/9/4
以纯化的番茄黄化曲叶病毒(TYLCV)外壳蛋白为抗原,免疫家兔制备并纯化出TYLCV的多克隆抗体IgG,以此抗体做包被抗体,并用碱性磷酸酶(AP)对其进行标记作为酶标抗体,从而建立了番茄黄化曲叶病毒的双抗体夹心ELISA(DAS-ELISA)检测方法。通过ELISA方阵试验确定该法的最佳工作浓度为酶标抗体(IgG-AP)作1:400倍稀释,包被抗体浓度为6.25μg ·mL-1;并且确定了抗原最低...
Detection and Characterization of Plum Pox Potyvirus（PPV）by DAS-ELISA and RT-PCR / RFLP Analysis in Turkey
Enzyme-linked immunosorbent assay plum pox potyvirus restriction analysis reverse transcription-polymerase chain reaction
2009/1/13
This study was conducted to determine the presence of plum pox virus (PPV) (family Potyviridae, genus Potyvirus) in different regions of Turkey and to characterize PPV isolates by serological and mol...
Detection of Four Apple Viruses by ELISA and RT-PCR Assays in Turkey
Apple virus RT-PCR ELISA Turkey
2009/1/12
Plant samples were collected from the main apple growing provinces of Turkey in order to evaluate the incidence of 4 important apple virus diseases during spring 2004. Collected leaves and shoots were...
以对硫磷为对象,通过与常规方法相比较,对均匀设计法在ELISA分析方法条件优化实验中应用的适用性进行了研究。采用常规方法对对硫磷ELISA分析方法条件优化的结果为:甲醇体积分数为5％,离子强度0.50 mol/L和pH 7.40,此时建立的ELISA方法IC50值为 89.17 ng/mL,IC20值为 12.49 ng/mL。而采用均匀设计法对对硫磷ELISA分析方法条件进行优化,并对实验结果进...