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Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D.1.Mutagenesis and Structural Studies
Arabidopsis thaliana homogentisate phytyltransferase (HPT/VTE2) Lactuca sativa tocopherol tocopherol cyclase (TC/VTE1) vitamin e.
2016/5/24
Wild type green fluorescent protein (wt-GFP) and the variant S65T/H148D each exhibit two absorption bands, A and B, which are associated with the protonated and deprotonated chromophores, respectively...
Discovery of New Ligand Binding Pathways in Myoglobin by Random Mutagenesis
Animals Whales Myoglobin Ligands Genetic Techniques Mutagenesis Binding Sites Amino Acid Sequence Protein Conformation Structure-Activity Relationship
2016/5/23
A random library of single amino acid mutants of myoglobin was generated using a highly efficient, single-base-misincorporation random mutagenesis method to discover new ligand-binding pathways in myo...
Photosynthetic Reaction Center Mutagenesis via Chimeric Rescue of a Non-functional Rhodobacter capsulatus puf Operon with Sequences from Rhodobacter sphaeroides
photosynthesis recombination bacteria energy transfer antenna
2016/5/23
Photosynthetically active chimeric reaction centers which utilize genetic information from both Rhodobacter capsulatus and Rb. sphaeroides puf operons were isolated using a novel method termed chimeri...
Characterization of a fatty acid elongase condensing enzyme by site-directed mutagenesis and biochemical analysis
ELONGASE CONDENSING ENZYME
2015/5/21
Fatty acid elongation is the extension of de novo synthesized fatty acids through a series of four reactions analogous to those of fatty acid synthase. ELOs catalyze the first reaction in the elongati...
Studies of the complex of ribosomal protein L1 with its binding site in 23S rRNA: Modification -interference, mutagenesis and crosslinking
Ribosomal proteins escherichia coli ribosomes the protein spiral protein
2014/12/11
Interaction of ribosomal protein L1 and the 23S rRNA plays an important role in both the structure and biological activity of the Escherichia coli ribosome. We have minimized the binding site for prot...
Aerobic Uptake of Cholesterol by Ergosterol Auxotrophic Strains in Candida glabrata & Random and Site-Directed Mutagenesis of ERG25 in Saccharomyces cerevisiae
Ergosterol Candida glabrata ERG25
2015/5/22
Candida albicans and Candida glabrata are opportunistic human pathogens that are the leading cause of fungal infections, which are increasingly becoming the leading cause of sepsis in immunosuppressed...
In vitro mutagenesis and identification of new variants via RAPD markers for improving Chrysanthemum morifolium
Chrysanthemum morifolium in vitro mutagenesis RAPD analysis somaclones
2011/1/11
The objective of this study was to induce mutation in Chrysanthemum morifolium cv. ‘Delistar White’ through in vitro mutagenesis by treating the ray florets with two doses of gamma irradiation (0.5 an...
Mutagenesis of Leuconostoc Mesenteroides and selection of Dextransucrase hyperproducing strains
Dextransucrase Dextran Leuconostoc mesenteroides
2010/1/6
Dextransucrase of Leuconostoc mesenteroides PTCC 1059 is an enzyme of industrial and medical interest that catalyzes the synthesis of a soluble dextran from sucrose. The mutant strains of Leuconostoc ...
北京师范大学分子生物学课件CHAPTER 6 Mutations, Mutagenesis and DNA Repair
北京师范大学 分子生物学 课件 Mutations Mutagenesis DNA Repair
2009/11/14
北京师范大学分子生物学课件CHAPTER 6 Mutations, Mutagenesis and DNA Repair。
The Effects of Vitamin C on Oxidative DNA Damage and Mutagenesis
antigenotoxic potential S. cerevisiae reversion assay yeast comet assay
2009/3/6
DNA damage induced by reactive oxygen species is involved in mutagenesis and generation of mutation-related diseases, including cancer. Therefore, study of antigenotoxic potential of antioxidants is o...
In vitro Mutagenesis for Genetic Improvement of Salinity Tolerance in Wheat
in vitro embryonic culture Wheat varieties gamma rays and salinity stress protein and isozyme markers
2010/11/11
Combining the effects of salinity stress and gamma rays showed eight of the variable bands were affected by gamma rays, whereas some were induced at different gamma doses
under 0.9 and 1.2% NaCl. Ele...
Improvement of the Enzymatic Properties of Kojibiose Phosphorylase from Thermoanaerobacter brockii by Random Mutagenesis and Chimerization
kojibiose phosphorylase random mutagenesis chimerization Thermoanaerobacter brockii
2008/4/20
Random mutagenesis by error-prone PCR was introduced to kojibiose phosphorylase (KP; EC 2.4.1.230) from Thermoanaerobacter brockii ATCC35047. One thermostable mutant and two DP-mutants that were defin...
Improvement of the Thermal Stability of a Calcium-free, Alkaline α-Amylase by Site-directed Mutagenesis
Bacillus alkaliphile α-amylase thermostability site-directed mutagenesis
2008/4/20
Alkaline α-amylase from Bacillus sp. strain KSM-K38 (AmyK38) is a calcium-free enzyme that is stable against chelating and oxidative reagents. Recently, the thermostability of this enzyme was improved...
Creation of a Novel Hydrolase by Site-directed Mutagenesis of Malto-oligosyltrehalose Synthase
trehalose malto-oligosyltrehalose synthase site-directed mutagenesis transglycosylation hydrolysis
2008/4/20
Malto-oligosyltrehalose synthase (EC 5.4.99.15, MTSase) catalyzes the conversion of α-1,4-glucan to glycosyltrehalose by forming an α,α-1,1-glucosidic linkage on the reducing side of the α-1,4-glucan....
Enhancing the Thermal Stability of Sucrose Phosphorylase from Streptococcus mutans by Random Mutagenesis
sucrose phosphorylase Streptococcus mutans random mutagenesis thermostable enzyme
2008/4/20
The thermal stability of sucrose phosphorylase (EC 2.4.1.7) from Streptococcus mutans was enhanced using random and site-directed mutageneses. Random mutagenesis studies revealed that eight single ami...