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Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography
recombinant antigen ELISA immunoblo
2015/1/20
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal bindi...
Affinity chromatography purification of urokinase with epichlorohydrin activated agarose matrix
epichlorohydrin agarose affinity chromatography
2009/4/28
Affinity chromatography purification of urokinase with epichlorohydrin activated agarose
matrix.
A new kind of electrophoretic affinity chromatography (EAC) for bioseparation was proposed,Separation by EAC was conducted in a multicompartment electrolyzer in which the affinity gel media were pac...
Characterization of boar sperm plasma membranes by two-dimensional PAGE and isolation of specific groups of polypeptides by anion exchange chromatography and lectin affinity chromatography
plasma membranes spermatozoa twodimensional
2009/2/19
High resolution, two-dimensional PAGE was used to characterize purified plasma membranes (PM) from boar spermatozoa. In addition to the abundance of polypeptides resolved, unusual features of PM dis...
Interaction of Sucrose Esters with Skim Milk Proteins as Characterized by Affinity Chromatography
sucrose ester binding to milk protein affinity chromatography
2008/3/22
The interaction of sucrose ester, P-1670 (70% pure palmitic acid monoester), with milk proteins was examined by affinity chromatography. The ester was covalently bound to 3-aminopropyl controlled-pore...
BORONIC ACIDS AS LIGANDS FOR AFFINITY CHROMATOGRAPHY
boronic acids borate boronate boronate affinity chromatography separation of glycoproteins
2007/12/13
A review on the principles and applications of boronic acids as affinity ligands for the chromatographic separation of carbohydrates, nucleic acid components, glycoproteins, and other small biomolecul...