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铁蛋白Ferritin原核表达和纯化及纳米颗粒胞外自组装
铁蛋白 原核表达 亲和层析 胞外自组装
2018/4/3
旨在使用原核表达系统表达幽门螺杆菌铁蛋白Ferritin，获得具有天然纳米结构的铁蛋白纳米颗粒，从而通过表面修饰等方法应用于疾病治疗和抗原呈递等研究。将铁蛋白氨基酸序列按照大肠杆菌常用密码子进行优化，合成重组铁蛋白基因并克隆至原核表达载体。使用镍柱进行亲和层析纯化重组蛋白，并在透析复性后利用透射电子显微镜对体外自组装纳米颗粒进行鉴定。结果显示，本研究成功构建了pET-32a-Ferritin原核表...
Serum ferritin as an indicator of iron status: what do we need to know
iron status, iron deficiency, ferritin, iron deficiency anemia, cutoffs, hepcidin, diagnostic test
2018/11/22
Determination of iron status in pregnancy and in young children is essential for both clinical and public health practice. Clinical diagnosis of iron deficiency (ID) through sampling of bone marrow to...
Serum ferritin as an indicator of iron status: what do we need to know?
iron status iron deficiency ferritin iron deficiency anemia cutoffs hepcidin diagnostic test
2018/11/21
Determination of iron status in pregnancy and in young children is essential for both clinical and public health practice. Clinical diagnosis of iron deficiency (ID) through sampling of bone marrow to...
Adjusting ferritin concentrations for inflammation: Biomarkers Reflecting Inflammation and Nutritional Determinants of Anemia (BRINDA) project
anemia ferritin inflammation iron deficiency meta-analysis nutritional assessment
2018/11/15
Background: The accurate estimation of iron deficiency is important in planning and implementing interventions. Ferritin is recommended as the primary measure of iron status, but interpretability is c...
Higher concentrations of serum iron and transferrin saturation but not serum ferritin are associated with cancer outcomes
Busselton Health Study cancer outcomes follow-up study iron risk factors
2018/12/14
Background: Although the carcinogenic potential of iron has been shown, evidence from observational studies that have linked serum iron variables and cancer outcomes has been inconsistent.
Aspirin intake and the use of serum ferritin as a measure of iron status
Aspirin serum ferritin myocardial infarction
2016/6/14
Atherosclerosis, a primary cause of myocardial
infarction (MI), is an inflammatory disease. Aspirin use lowers
risk of MI, probably through antithrombotic and antiinflammatory
effects. Because seru...
HIV and other predictors of serum folate, serum ferritin, and hemoglobin in pregnancy: a cross-sectional study in Zimbabwe
Hemoglobin ferritin folate
2016/6/13
Folate and iron status and hemoglobin concentrations
are important to maternal and infant health.
Manganese absorption and retention by young women is associated with serum ferritin concentration
Manganese ferritin iron women
2016/5/31
The interaction between iron and manganese in
the gut is well characterized but iron status has not been shown
to affect manganese absorption.
School-administered weekly iron-folate supplements improve hemoglobin and ferritin concentrations in Malaysian adolescent girls
Iron nutrition iron-folate supplements effectiveness,
2016/5/31
Iron deficiency and its consequent anemia constitute
the commonest micronutrient deficiency in the world.
Serum ferritin and risk of myocardial infarction in the elderly: the Rotterdam Study
Serum ferritin iron status ischemic heart disease
2016/5/31
Elevated body iron stores have been suggested to
be a risk factor for ischemic heart disease.
Nonheme-iron absorption, fecal ferritin excretion, and blood indexes of iron status in women consuming controlled lactoovovegetarian diets for 8 wk
Nonheme-iron absorption bioavailability iron status
2016/5/31
The characteristics of vegetarian diets suggest
that these diets would have lower dietary iron bioavailability
than nonvegetarian diets, but there is no evidence of iron deficiency
in vegetarians.
Relationship Between Serum Hepcidin and Ferritin Levels in Patients With Thalassemia Major and Intermedia in Southern Iran
Hepcidin Hydroxyurea Ferritin Thalassemia Intermedia Thalassemia Major
2015/11/9
Background: Hepcidin is a key regulator of iron absorption in humans. It is mainly affected by hypoxia and iron stores.
Serum Ferritin Levels Correlation With Heart and Liver MRI and LIC in Patients With Transfusion-Dependent Thalassemia
Beta-thalassemia Ferritin Magnetic Resonance Imaging Liver Heart
2015/10/22
Background: Iron-loaded cardiac complication is the essential cause of mortality in patients with thalassemia. Early detection and treatment of cardiac over-load can reduce mortality.
Do High Blood Hepcidin Concentrations Contribute to Low Ferritin Levels in Young Tennis Players at the End of Tournament Season?
Pro-inflammatory cytokine overreaching hepcidin
2015/5/15
The purpose of the present study was to verify whether impaired iron metabolism in young athletes is a consequence of an excessive workload during the tournament season. Low levels of ferritin (under ...
Association between Serum Ferritin and Body Composition in Young Women
serum ferritin body composition
2014/11/7
I would like to thank my advisor Dr Alayne Ronnenberg for her valuable guidance,
support and motivation throughout the project.
I would also like to thank my committee members Dr Richard Wood, Dr ...