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中国科学院的新型基因编辑工具脱靶检测方法GOAT证明CRISPR-Cas12a不存在明显的脱靶效应（图）
新型 基因编辑 脱靶检测方法 GOAT证明 CRISPR-Cas12a 脱靶效应
2021/4/7
2021年4月2日，《Protein & Cell》发表了一篇名为《Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos》的研究论文，该研究由中科院脑科学与智能技术卓越创新中心（神经科学研究所）、上海脑科学与类脑研究中心周昌阳...
Molecular characterization and expression pattern of a novel Keratin-associated protein 11.1 gene in the Liaoning cashmere goat (Capra hircus)
Liaoning Cashmere Goat Keratin-associated Protein 11.1 (KAP11.1) Expression Pattern
2017/3/9
An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness.
Effects of Weaning and Spatial Enrichment on Behavior of Turkish Saanen Goat Kids
Environmental Enrichment Turkish Saanen Growth Bipedal Animal Welfare
2016/7/27
As is in all economic activities, the highest yield per unit area is the main goal in animal production, while addressing the temperamental needs of animals often is ignored. Animal welfare is not onl...
Characteristics and Gel Properties of Gelatin from Goat Skin as Influenced by Alkaline-pretreatment Conditions
Goat Gelatin Skin Pretreatment Alkaline Gel Strength
2016/7/27
Characteristics and properties of gelatin from goat skin pretreated with NaOH solutions (0.50 and 0.75 M) for various times (1 to 4 days) were investigated. All gelatins contained α-chains as the pred...
Quality Enhancement of Edam-Like Cheese Made From Goat’s Milk
Made From Goat’s Milk Edam-Like
2016/6/2
In the present work, Edam-like cheese was made from Zaraibi goats’ milk. The milk originated from goats fed on diets supplemented with low canola seeds (LCS, 5%) and high canola seeds (HCS, 10%), low ...
Cloning and Characterization of an Endoglucanase Gene from Actinomyces sp. Korean Native Goat 40
Korean Native Goat Actinomyces sp Endo-β-1 4-glucanase Cellulase
2016/5/20
A gene from Actinomyces sp. Korean native goat (KNG) 40 that encodes an endo-β-1,4-glucanase, EG1, was cloned and expressed in Escherichia coli (E. coli) DH5α. Recombinant plasmid DNA from a positive ...
Combination of let-7d-5p,miR-26a-5p,and miR-15a-5p is suitable normalizer for studying microRNA expression in skin tissue of Liaoning cashmere goat during hair follicle cycle
cashmere goat skin microRNA expression reference expression stability
2016/3/24
The microRNAs are non-coding RNA molecules of approximately 20–22 nucleotides that are found to be implicated in a wide range of physiological processes. In this study, the suitability of 10 candidate...
Tissue-specific mRNA expression profiles of GDF9,BMP15,and BMPR1B genes in prolific and non-prolific goat breeds
goat prolificacy ovary RT-PCR gene expression
2015/10/29
The tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in goats were investigated. The mRNA expression of the genes was detected in prolific Jining Grey goats and non-prolific L...
Effect of sodium selenite or lactate-protein selenium complex supplementation on selenium status in goat kids
organic selenium inorganic selenium
2015/7/7
The aim of the study was to compare the effect of selenium (Se) supplementation in organic
(lactate-protein complex) or inorganic (sodium selenite) forms on Se concentrations in the blood and organs...
Nucleotide sequencing and DNA polymorphism studies of beta-lactoglobulin gene in native Saudi goat breeds in relation to milk yield
Saudi goats functional polymorphism
2015/7/7
β-Lactoglobulin (β-LG) is the dominant non-casein whey protein found in milk of bovine and of
most ruminants. The amino acid sequence of β-LG along with its 3-dimensional structure illustrates linka...
Expression of goat RNA binding protein (NANOS2), development and evaluation of anti-NANOS2 IgY antibodies
NANOS2 protein dairy goat
2015/7/7
. Anti-NANOS2 immunoglobulin Y
(IgY; in chicken) was developed and evaluated. NANOS2 protein sequences between mice and dairy goat were
compared using bioinformatics tools. The specific IgY t...
Copper metabolism in goat杒id relationship at supplementation of inorganic and organic forms of copper
trace elements organic copper inorganic copper
2015/7/2
The aim of the experiment was to compare the effect of inorganic and organic forms of copper (Cu) supplementation on Cu status of goats and their kids, colostrum and milk composition and quality, and ...
Goat mastitis detection using daily records of milk conductivity: comparative results of different algorithms
intramammary infection gland measurements on-line
2015/7/2
Milk electrical conductivity is employed for mastitis detection in cows due to its automation, low cost, and infection detectability at early stage. Nevertheless, the number of publications about its ...
Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traits
milk yield fat percentage dairy goat SNP PCR-RFLP SSCP additive effect
2015/6/24
Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g.407_408insC in the...
Intestinal histology of newborn goat kids fed lyophilized bovine colostrum
lacteal secretion intestinal morphometry goblet cells newborn kids stereology
2015/6/24
Enteric histology of newborn goat kids fed lyophilized bovine colostrum (LBC) was studied. At 0, 7, and 14 h of life 15 male newborns received 5% of body weight of lyophilized bovine colostrum and 14 ...