搜索结果: 1-12 共查到“林学 小RNA”相关记录12条 . 查询时间(0.063 秒)
Analysis of Arabidopsis seedling responses to daytime temperature regimes identifies an mRNA hairpin as a novel thermosensor in plants.
Protocol: low cost fast and efficient generation of molecular tools for small RNA analysis
Vector Target mimic miRNA, Cloning Plant Downregulation
2024/1/19
Small RNAs are sequence-dependent negative regulators of gene expression involved in many relevant plant processes such as development, genome stability, or stress response. Functional characterizatio...
Live single-cell transcriptional dynamics via RNA labelling during the phosphate response in plants
RNA single-cell transcriptional dynamics phosphate response plant development
2023/12/1
Plants are constantly adapting to ambient fluctuations through spatial and temporal transcriptional responses. Here, we implemented the latest-generation RNA imaging system and combined it with microf...
A Picea wilsonii NAC transcription factor, PwNAC1, interacts with RNA-binding protein PwRBP1 and synergistically enhances drought and salt tolerance of transgenic Arabidopsis
Picea wilsonii Transcription factor PwNAC1 PwRBP1 Abiotic stress ABA signaling
2023/11/16
NAC transcription factors play crucial roles in plant resistance to a variety of adverse circumstances. Here, we characterized a NAC transcription factor PwNAC1 from Picea wilsonii and subcellular loc...
Two environmental signal-driven RNA metabolic processes: alternative splicing and translation
alternative splicing environmental signal receptor kinase RNA metabolism
2023/11/16
Plants live in fixed locations and have evolved adaptation mechanisms that integrate multiple responses to various environmental signals. Among the different components of these response pathways, rec...
林木遗传育种国家重点实验室林木基因组定向选择育种研究组在长链非编码RNA参与杨树生长和苯丙氨酸通路的研究取得新进展。
杨树作为我国重要的造林、生态和能源树种，长链非编码RNA（lncRNA）可以在转录、转录后和表观遗传学水平上调节基因表达，因此挖掘lncRNA在生长和木材品质中的作用具有重要意义。
近日，国际植物学TOP期刊《Plant Biotechnology Journal》（IF: 7.443, 植物学一区）发表我校林木分子育种创新团队研究成果，题目是“Osmotic stress-responsive promoter upstream transcripts (PROMPTs) act as carriers of MYB transcription factors to ind...
北京林业大学全面解析林木非编码小RNA
非编码小RNA 育种
2016/7/21
北京林业大学张德强团队在前期创制林木基因标记辅助育种理论体系的基础上，首次系统开展了林木非编码小RNA功能进化与等位变异的联合解析工作，并取得系列进展。
银杏叶片RNA提取方法的研究
银杏叶片 RNA提取方法
2011/9/1
为了获得一种银杏（Ginkgo biloba L.）叶片总RNA提取的理想方法，为后续的分子生物学研究打下基础，笔者以不同生长期的叶片为材料，利用生物分光光度计和凝胶电泳法比较了改良CTAB法、乙二醇丁醚法以及改良Trizol法提取的银杏总RNA的产率和纯度。结果表明：改良Trizol法所提取RNA的OD260/OD280比值介于1.8~2.0之间，并且OD260/OD230在1.9~2.1之间，...
血红鸡爪槭叶片总RNA提取方法的比较研究
血红鸡爪槭 总RNA提取 RT-PCR
2011/9/5
红鸡爪槭叶片中富含多糖、多酚物质，严重影响了RNA提取纯度和质量。为了获得适宜血红鸡爪槭叶片总RNA提取的方法，采用柱式植物RNAout法、TRIZOL法和改良CTAB法3种提取方法对其叶片总RNA提取效果进行比较分析。结果显示，采用柱子法提取获得的总RNA产率最高，但是有DNA污染；TRIZOL法提取的总RNA OD260∕OD280为1.65，可能有多糖和酚类物质的污染，并且产率最低；改良CT...
RNA干扰机理与应用
RNAi PTGS 机制 应用
2009/7/3
RNA 干扰( RNA Interference ,RNAi) 是一种转录后水平的基因沉默( Post-transcriptional Gene Silencing ,PTGS) 现象, 是通过体外人工合成的或体内的双链RNA( Dubble- stranded RNA,dsRNA) 在细胞内特异性的降解其同源mRNA, 使相应的基因沉默, 达到阻止基因表达的目的。因其具有特异、高效、易于操作等优...