搜索结果: 1-15 共查到“农学 RNA”相关记录269条 . 查询时间(0.211 秒)
本发明公开了分子生物学技术领域的一种果树中高糖多酚类果实组织RNA的提取方法,包括以下步骤:(1)、取100-150mg粉末状的果实样品至液氮提前预冷的离心管内,立即加入1000μL 2×CTAB提取缓冲液和30μL2%β-巯基乙醇,涡旋震荡混匀,65℃水浴10min;(2)、向步骤(1)离心管中加入与提取缓冲液等体积、体积比为24:1的氯仿和异戊醇混合液,冰浴10min,充分颠倒混匀3-5次,于...
Analysis of Arabidopsis seedling responses to daytime temperature regimes identifies an mRNA hairpin as a novel thermosensor in plants.
Protocol: low cost fast and efficient generation of molecular tools for small RNA analysis
Vector Target mimic miRNA, Cloning Plant Downregulation
2024/1/19
Small RNAs are sequence-dependent negative regulators of gene expression involved in many relevant plant processes such as development, genome stability, or stress response. Functional characterizatio...
近日,植物保护学院作物病原真菌功能基因组学研究团队在《Science Advances》上在线发表题为“Adaptive advantages of restorative RNA editing in fungi for resolving survival-reproduction trade-offs”的论文,齐朝妹博士为论文第一作者,刘慧泉教授为通讯作者。
Live single-cell transcriptional dynamics via RNA labelling during the phosphate response in plants
RNA single-cell transcriptional dynamics phosphate response plant development
2023/12/1
Plants are constantly adapting to ambient fluctuations through spatial and temporal transcriptional responses. Here, we implemented the latest-generation RNA imaging system and combined it with microf...
西北农林科技大学专利:一种植物抗病毒RNA沉默相关转录因子筛选方法及应用
西北农林科技大学 植物抗病毒 RNA沉默 转录因子
2023/11/24
本发明公开了一种植物抗病毒RNA沉默相关转录因子筛选方法及应用,以植物矮牵牛作为研究对象,采用转录组测序和VIGS相结合的技术,首先构建TRV病毒侵染的差异表达基因数据库,从中选择极显著差异表达转录因子作为候选基因;分别构建至TRV?GFP?PhPDS载体,利用双报告基因分别监测病毒积累与RNA沉默过程;接种矮牵牛后,以绿色荧光和光漂白表型作为筛选依据,在上调表达转录因子中,导致绿色荧光增强、光漂...
非编码RNA介导的宿主抗性反应机理
非编码 RNA 植物免疫 抗性反应
2023/12/27
A Picea wilsonii NAC transcription factor, PwNAC1, interacts with RNA-binding protein PwRBP1 and synergistically enhances drought and salt tolerance of transgenic Arabidopsis
Picea wilsonii Transcription factor PwNAC1 PwRBP1 Abiotic stress ABA signaling
2023/11/16
NAC transcription factors play crucial roles in plant resistance to a variety of adverse circumstances. Here, we characterized a NAC transcription factor PwNAC1 from Picea wilsonii and subcellular loc...
Two environmental signal-driven RNA metabolic processes: alternative splicing and translation
alternative splicing environmental signal receptor kinase RNA metabolism
2023/11/16
Plants live in fixed locations and have evolved adaptation mechanisms that integrate multiple responses to various environmental signals. Among the different components of these response pathways, rec...
中国农业科学院植物保护研究所研究揭示正单链RNA病毒编码小蛋白新策略(图)
正单链 RNA 病毒 蛋白编码
2023/11/15
2023年9月22日,西南大学植物保护学院丁伟教授团队在期刊International Journal of Biological Macromolecules(中科院1区TOP,IF=8.2)在线发表研究论文《Chitosan/dsRNA polyplex nanoparticles advance environmental RNA interference efficiency throug...
近日,南京农业大学园艺学院王长泉教授团队在Plant Physiology杂志发表了题为“Rose long non-coding RNA lncWD83 promotes flowering by modulating ubiquitination of the floral repressor RcMYC2L”的研究成果,该研究从月季Rosa chinensis‘Old Blush’...